General Information

  • ID:  hor004121
  • Uniprot ID:  P13049
  • Protein name:  Leukopyrokinin
  • Gene name:  NA
  • Organism:  Rhyparobia maderae (Madeira cockroach) (Leucophaea maderae)
  • Family:  Pyrokinin family
  • Source:  animal
  • Expression:  NA
  • Disease:  NA
  • Comments:  NA
  • Taxonomy:  Rhyparobia (genus), Oxyhaloinae (subfamily), Blaberidae (family), Blaberoidea (superfamily), Blattodea (order), Dictyoptera (superorder), Polyneoptera (cohort), Neoptera (infraclass), Pterygota (subclass), Dicondylia, Insecta (class), Hexapoda (subphylum), Pancrustacea, Mandibulata, Arthropoda (phylum), Panarthropoda, Ecdysozoa, Protostomia, Bilateria, Eumetazoa, Metazoa (kingdom), Opisthokonta, Eukaryota (superkingdom), cellular organisms
  • GO MF:  NA
  • GO BP:  GO:0007218 neuropeptide signaling pathway
  • GO CC:  GO:0005576 extracellular region

Sequence Information

  • Sequence:  QTSFTPRL
  • Length:  8(1-8)
  • Propeptide:  QTSFTPRL
  • Signal peptide:  NA
  • Modification:  T1 Pyrrolidone carboxylic acid;T8 Leucine amide
  • Glycosylation:  NA
  • Mutagenesis:  NA

Activity

  • Function:  Mediates visceral muscle contractile activity
  • Mechanism:  An analog without the N-terminal PCA residue was synthesized and found to exhibit greater activity (144%) than the parent neuropeptide. The portion of the sequence of LPK most critical for the myotropic properties is limited to the pentapeptide fragment FTPRL.
  • Cross BBB:  NO
  • Target:  NA
  • Target Unid:  NA
  • IC50: NA
  • EC50: NA
  • ED50: NA
  • kd: NA
  • Half life: NA

Structure

  • Disulfide bond:  NA
  • Structure ID:  NA

  • Structure: (PDB_ID-from https://www.rcsb.org/; AlphaFold_DB_ID-from https://alphafold.ebi.ac.uk/; hordbxxxxxx_AF2.pdb was predicted structure by AlphaFold2; hordbxxxxxx_ESM.pdb was predicted structure by ESMFold)
    •    hor004121_AF2.pdbhor004121_ESM.pdb

Physical Information

Mass: 107410 Formula: C42H68N12O13
Absent amino acids: ACDEGHIKMNVWY Common amino acids: T
pI: 10.55 Basic residues: 1
Polar residues: 3 Hydrophobic residues: 2
Hydrophobicity: -65 Boman Index: -2110
Half-Life: 0.8 hour Half-Life Yeast: 10 min
Half-Life E.Coli: >10 hour Aliphatic Index 48.75
Instability Index: -67.5 Extinction Coefficient cystines: 0
Absorbance 280nm: 0

Literature

  • PubMed ID:  3015140
  • Title:  Active fragments and analogs of the insect neuropeptide leucopyrokinin: structure-function studies.
  • PubMed ID:  2877794
  • Title:  Primary structure and synthesis of a blocked myotropic neuropeptide isolated from the cockroach, Leucophaea maderae.